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KMID : 1161519980020020233
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Animal Cells and Systems
1998 Volume.2 No. 2 p.233 ~ p.238
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Characteristics of (3H)-choline uptake into synaptosomes from rat hippocampus
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Lee Seo-Eun
Hong Seung-Kil
Choe Rim-Soon
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Abstract
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Certain basic characteristics of choline uptake in nerve terminals were studied with synaptosomes from rat hippocampus. Synaptosomal [3H]?choline uptake was clarified as specific and high affinity by low Km value (2.2 μM), Na+?dependency and high sensitivity to hemicholinium?3, a competitive inhibitor of choline uptake. Choline uptake into synaptosomes was linearly related to Na+ concentration and membrane potential. Extracellular Ca2+ modulated the choline uptake, but probably not through increase of intracellular Ca2+ because this modulation was not affected the by high K+?depolarization. EGTA (2 mM) added for Ca2+ ?free condition had a peculiar effect of decreasing choline uptake. These results suggest that Ca2+ may play an important role in regulating the metabolism of acetylcholine in the nerve terminals directly through the increase of acetylcholine release
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KEYWORD
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Calcium, Choline uptake, High affinity, Na+-dependent, Synaptosomes
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